Characterization of novel marine bacterium vitellibacter aquimaris using polyphasic approach and genomic analyses
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Date
2018
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Universiti Teknologi Malaysia
Abstract
The discovery of novel marine bacteria provides an opportunity in exploring new bioactive compounds and their significance. Extensive industrial applications of enzymes promote the search of the enzymes from the new source. In this study, a yellow-orange strain designated as D-24 was isolated from Desaru, Johor. The study aimed to taxonomically and genomically characterize strain D-24 through polyphasic approach and genome analyses, respectively. The 16S rRNA gene sequence of strain D-24 indicated that it belongs to genus Vitellibacter. Strain D-24 was distinct from the other Vitellibacter spp. in hydrolyse Tween 60 and tyrosine, composition of fatty acids, polar lipid profile and DNA–DNA relatedness. Hence, strain D-24 was proposed as a new type strain with the name of Vitellibacter aquimaris. Following, strain D-24 was characterized on its protease activity and extracellular protease encoding genes. The characterization of crude protease demonstrated optimum activity at 60 °C, 5 % (w/v) NaCl and pH 7. The draft genome sequence of V. aquimaris (3.1 Mbp) was generated using an Illumina MiSeq sequencer. Mining of genes revealed the presence of three metalloproteases, two serine proteases and a cysteine protease. Recently, genus Vitellibacter was suggested as the same genus of Aequorivita based on phylogenomic data with no further analyses. Therefore, the detailed comparative analyses were performed based on their phenotypic and genomic features. Analyses of 16S rRNA gene sequences, housekeeping genes, percentage of conserved proteins and phenotypic features supported both of them as same genus. Furthermore, the presence of proteases, glycoside hydrolases and denitrification genes in genome of Vitellibacter/Aequorivita revealed their role in detergent industry, food industry and in carbon mineralization. In a nutshell, this study has provided new knowledge on V. aquimaris and also the useful genes such as serine protease, beta-mannosidase and nitrous oxide reductase that could be further characterized
Description
Thesis (PhD. (Biosciences))
Keywords
Biosciences and medical engineering