dc.contributor.author	Abdullah, Norhanisah
dc.date.accessioned	2024-05-20T06:43:48Z
dc.date.available	2024-05-20T06:43:48Z
dc.date.issued	2017
dc.description	Thesis (PhD. (Bioprocess Engineering))
dc.description.abstract	Labisia pumila (L. pumila) is widely reported to exhibit antioxidant, antimicrobial and anti-cancer activities. The goal of this study was to identify and quantify triterpenoid compounds from L. pumila leaves which are responsible for anti-inflammatory and anti-elastase activities by using bioassay-guided fractionation. The leaves of L. pumila were sequentially extracted with n-hexane, ethyl acetate, methanol, and water using serial exhaustive extraction procedure. Qualitative phytochemical analysis revealed the presence of phenolics, flavonoids, terpenoids, cardiac glycosides and saponins in the extracts. Each extract was assessed for total triterpenoids and polyphenols content by using vanillin-perchloric acid assay and Folin-Ciocalteu’s reagent, respectively. The ethyl acetate extract has the highest total triterpenoids content (78.94 mg oleanolic acid equivalent (OAE)/g dried extract) and appreciable amount of total polyphenols (38.93 mg gallic acid equivalent/g dried extract). The extracts were then tested for their anti-inflammatory and anti-elastase activities. The ethyl acetate extract showed the highest anti-inflammatory activity via the inhibition of cyclooxygenase-2 (half-maximal inhibitory concentration (IC50) of 34.32 µg/ml) and elastase (IC50 of 17.14 µg/ml). The correlation analysis of antiinflammatory and anti-elastase ability of L. pumila with its total triterpenoids and total polyphenols content showed positive relationship. Further fractionation of the ethyl acetate extract by solid phase extraction afforded sample fraction EAF-B with high total triterpenoids content (91.50 mg OAE/g dried fraction) and anti-elastase activity (IC50 of 13.27 µg/ml). Rapid liquid chromatography-electrospray ionizationtandem mass spectrometry method was developed and validated for the identification and quantification of pentacyclic triterpenoids with elastase inhibition property from the sample fraction EAF-B. Three pentacyclic triterpenoids, namely betulinic acid, oleanolic acid and lupeol were identified. The content of betulinic acid, oleanolic acid and lupeol in the fraction were 2.67, 5.04 and 1.47 mg/g dried fraction, respectively. The limits of detection and quantification ranged from 0.04 to 0.12 µg/ml and 0.11 to 0.65 µg/ml, respectively. The response surface methodology analysis showed that solvent-solid ratio (p<0.01), extraction time (p<0.05) and temperature (p<0.01) were statistically significant factors affecting the total triterpenoids extraction. The optimum extraction conditions obtained were: solventsolid ratio of 37.76, extraction time of 4.24 hours and extraction temperature of 55.27 °C. The results suggested that L. pumila has promising anti-inflammatory and antielastase activity that could serve as potential source of natural elastase inhibitor in treating inflammatory skin disorders and photoaging
dc.description.sponsorship	Faculty of Chemical Engineering
dc.identifier.uri	http://openscience.utm.my/handle/123456789/1175
dc.language.iso	en
dc.publisher	Universiti Teknologi Malaysia
dc.subject	Plant bioactive compounds
dc.subject	Phytochemicals—Biotechnology
dc.subject	Botanical chemistry
dc.title	Elastase and inflammation inhibitory from labisia pumila
dc.type	Thesis
dc.type	Dataset

Elastase and inflammation inhibitory from labisia pumila

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